| First Author | Akao Y | Year | 1996 |
| Journal | Cytogenet Cell Genet | Volume | 75 |
| Issue | 1 | Pages | 38-44 |
| PubMed ID | 8995487 | Mgi Jnum | J:37353 |
| Mgi Id | MGI:84754 | Doi | 10.1159/000134454 |
| Citation | Akao Y, et al. (1996) Identification and chromosome mapping of the mouse homologue of the human gene (DDX6) that encodes a putative RNA helicase of the DEAD box protein family. Cytogenet Cell Genet 75(1):38-44 |
| abstractText | The human DDX6 gene (alias RCK) at chromosome some 11 band q23 was identified through the study of the breakpoint of t(11;14)(q23;q32) translocation in a B-cell lymphoma cell liner RC-K8, DDX6 encodesa DEAD box protein/RNA RNA helicase, Positive mouse genomic and cDNA recombinant clones were obtained by screening mouse B-tell genomic and cDNA libraries with a human DDX6 cDNA probe, The deduced amino acid sequence of an open reading frame from a cDNA clone revealed a protein with 92.5% identity to human ddx6/p54. All positive mouse genomic recombinant clones, and cDNA clones containing mouse Ddx6 (previous gene symbol: Rck), were localized by fluorescent in situ hybridization to band B of mouse Chromosome 9, a region showing conserved linkage homology to human chromosome 11 band q23. Mouse Ddx6 was localized to the region between Ncam and D9Mit45 by molecular linkage analysis, A 7.5-kb mRNA and a 54-kDa protein were identified as mouse Ddx6 gene products which are similar in size to products of the human DDXG gene, as shown by Northern and Western blot analyses. |
