| First Author | Silins G | Year | 1997 |
| Journal | Biochem Biophys Res Commun | Volume | 230 |
| Issue | 2 | Pages | 413-8 |
| PubMed ID | 9016794 | Mgi Jnum | J:37893 |
| Mgi Id | MGI:85288 | Doi | 10.1006/bbrc.1996.5979 |
| Citation | Silins G, et al. (1997) Analysis of the promoter region of the human VEGF-related factor gene. Biochem Biophys Res Commun 230(2):413-8 |
| abstractText | We have characterised the promoters of the human and murine VRF (vascular endothelial growth factor (VEGF) related factor) gene. A series of deletions were made of a 553-bp region 5' of the VRF initiation codon and were used in a luciferase reporter gene assay to determine the minimal promoter of the VRF gene. The region between base pairs -443 and -195 was sufficient to mediate transcription in lymphocytes and the region between -550 and -443 enhanced this promoter activity. Primer extension studies identified two regions of transcription initiation, both of which are preceded by Sp1, AP-2 and Egr-1 transcription factor binding sites. The VRF promoter is similar to VEGF in that it is associated with a CpG island, contains sites for Sp1 and AP-2, and lacks a TATA box. However, it has marked differences in that the promoter contains Egr-1 sites and lacks both hypoxia-inducible factor-1 and AP-1 sites. These data may indicate that expression of these two growth factors is regulated by different physiological stimuli. |
