| First Author | McMahan CJ | Year | 1997 |
| Journal | J Immunol | Volume | 158 |
| Issue | 5 | Pages | 2202-10 |
| PubMed ID | 9036966 | Mgi Jnum | J:38505 |
| Mgi Id | MGI:85888 | Doi | 10.4049/jimmunol.158.5.2202 |
| Citation | McMahan CJ, et al. (1997) Definition of a large region of RAG1 that is important for coimmunoprecipitation of RAG2. J Immunol 158(5):2202-10 |
| abstractText | Interaction between the RAG1 and RAG2 proteins is probably critical for V(D)J recombination. Using a coimmunoprecipitation assay, we define a large region of RAG1 (amino acids 504-1008) that is sufficient for interaction with RAG2. This region comprises the C- terminal half of the RAG1 protein, and is within the region defined as the recombinationally active core. Deletion of either of two regions of RAG1 (amino acids 504- 570 or 850-1008) causes a loss of interaction with RAG2. Loss of coimmunoprecipitation is also seen with RAG1 core proteins containing deletions of smaller stretches of amino acids (amino acids 506-511 or 545-550), emphasizing the importance of this region of RAG1 in forming a complex with RAG2. A variety of other small deletion mutations within the amino acid region 504-1008 also decrease coimmunoprecipitation of RAG2 with RAG1, indicating that much or all of this region is important for complex formation. |
