| First Author | Jippo T | Year | 1997 |
| Journal | Am J Pathol | Volume | 150 |
| Issue | 4 | Pages | 1373-82 |
| PubMed ID | 9094993 | Mgi Jnum | J:39284 |
| Mgi Id | MGI:86667 | Citation | Jippo T, et al. (1997) Expression of mast-cell-specific proteases in tissues of mice studied by in situ hybridization. Am J Pathol 150(4):1373-82 |
| abstractText | The pretense mRNA expression phenotype of individual mast cells was studied by in situ hybridization, Mouse mast cell pretense (MMCP)-2 mRNA teas expressed by mast cells located in the mucosa of the stomach of WB-(+/+) and (WB x C57BL/6) F-1-+/+ (hereafter WBB6F(1)-+/+) mice but not by mast cells in the same tissue of C57BL/6-+/+ mice. Even in the stomach of WBB6F(1)-+/+ mice, mast cells located in the muscularis propria did not express MMCP-2 mRNA, The mRNAs of MMCP-4 and mouse mast cell carboxypeptidase A were not expressed by mast cells in the stomach mucosa of untreated WBB6F(1)-+/+ mice but were expressed after the infection of Strongyloides venezuelensis. We examined whether MMCP-2 mRNA expression varied by changing environments of mast cells. Cultured mast cells of WBB6F(1)-+/+ mice that expressed MMCP-2 mRNA were transplanted into the stomach wall of genetically mast- cell-deficient WBB6F(1)-W/W-nu mice, Mast cells that appeared ill the mucosa expressed the MMCP-2 mRNA, but mast cells that appeared in the muscularis propria did not, indicating the adaptation of cultured mast cells into a new environment. In contrast to cultured mast cells, peritoneal mast cells of WBB6F(1)-+/+ mice that expressed MMCP-2 mRNA as well did not adapt to the muscularis propria of WBB6F(1)-W/W-nu mice. The MMCP-2 mRNA remained to be expressed after the settlement ill either the mucosa or the muscularis propria. Furthermore, the peritoneal mast cells did not change the MMCP-4 and MMCP-6 mRNA expression phenotype after the settlement in either the mucosa or the muscularis propria of WBB6F(1)-W/W-nu mice. The present result indicated that both intracellular factors such as strain specificity and source of mast cells and extracellular factors such as tissue specificity and helminth infection influenced the pretense expression phenotypes. |
