| First Author | Sugiura K | Year | 1997 |
| Journal | Biochim Biophys Acta | Volume | 1352 |
| Issue | 1 | Pages | 23-6 |
| PubMed ID | 9177479 | Mgi Jnum | J:40214 |
| Mgi Id | MGI:87556 | Doi | 10.1016/s0167-4781(97)00045-6 |
| Citation | Sugiura K, et al. (1997) Expression cloning and intracellular localization of a human ZF5 homologue. Biochim Biophys Acta 1352(1):23-6 |
| abstractText | We isolated a cDNA encoding a human homologue of ZF5 (hZF5), which has five Kruppel-like C2H2 type zinc fingers at carboxyl terminus and the BTB/POZ (poxvirus and zinc finger) at the amino terminus, using autoimmune sera from a patient with overlap syndrome (dermatomyositis and scleroderma). Sequencing of the entire cDNA revealed an open reading frame (ORF) of 1349 bp with a deduced protein sequence of 449 amino acid residues and a calculated molecular weight of 51.3 kDa. The deduced amino acid sequence of hZF5 is highly homologous to mouse ZF5 (99.3% identity). Immunofluorescence studies revealed that HA-tagged hZF5 transiently expressed in COS-7 cells showed the nuclear dot pattern in the BTB/POZ domain-dependent manner. |
