| First Author | Lamb KA | Year | 1997 |
| Journal | Mol Reprod Dev | Volume | 48 |
| Issue | 3 | Pages | 301-9 |
| PubMed ID | 9322240 | Mgi Jnum | J:43321 |
| Mgi Id | MGI:1097501 | Doi | 10.1002/(SICI)1098-2795(199711)48:3<301::AID-MRD1>3.0.CO;2-V |
| Citation | Lamb KA, et al. (1997) NF-Y binds to the CCAAT box motif of the FGF-4 gene and promotes FGF-4 expression in embryonal carcinoma cells. Mol Reprod Dev 48(3):301-9 |
| abstractText | FGF-4 appears to be the first fibroblast growth factor (FGF) expressed during embryogenesis, and its expression is critical for early mammalian development. FGF-4 is expressed in the embryonic cell lines, F9, D3, and NT2/D1; but its expression in these cells is repressed upon differentiation. Transcription of the FGF-4 gene in embryonic cells is regulated by an enhancer in the third exon and by a positive regulatory region upstream of the transcription start site. A CCAAT box motif within the positive regulatory region has been shown to support FGF-4 expression, but the factor that binds to this site in vivo has not been identified. In this report, we demonstrate that the transcription factor complex NF-Y binds to the FGF-4 CCAAT box motif when nuclear extracts from each of the embryonic cell lines and their differentiated cells were examined by gel mobility shift analyses. Importantly, we demonstrate that expression of a dominant-negative NF-YA mutant protein reduces the expression of FGF-4 promoter/reporter gene constructs in F9 EC cells. Hence, we provide strong evidence that the transcription factor NF-Y is involved in the expression of the FGF-4 gene. |
