| First Author | Morris DW | Year | 1997 |
| Journal | Virology | Volume | 238 |
| Issue | 1 | Pages | 161-5 |
| PubMed ID | 9375020 | Mgi Jnum | J:44691 |
| Mgi Id | MGI:1100817 | Doi | 10.1006/viro.1997.8794 |
| Citation | Morris DW, et al. (1997) Identification of a MMTV insertion mutation within the coding region of the Fgf-3 protooncogene. Virology 238(1):161-5 |
| abstractText | The Fgf-3 protooncogene (previously called int-2) is a target of proviral insertion mutations in mammary tumors induced by the mouse mammary tumor virus (MMTV). These insertion mutations result in the transcriptional activation of Fgf-3, which is not normally expressed in the adult mammary gland. Previous mapping studies of numerous Fgf-3 insertion mutations have failed to reveal any provirus integrations within the gene coding region. This finding is consistent with the hypothesis that oncogenesis occurs in this system as a consequence of up-regulation of Fgf-3 transcription, rather than from alterations of the gene product. During an analysis of a new cohort of tumors from the WXG-2 mouse strain, a breast tumor was identified which had a MMTV provirus integrated 24 bp upstream of the Fgf-3 stop codon. This insertion mutation generated a fusion transcript which was readily detectable in tumor RNA by RT-PCR. The predicted protein product of this fusion transcript is missing 8 aa of native sequence and contains an additional 8 aa of cryptic MMTV-encoded sequence. These data document the first exception to the generalization that the Fgf-3 coding region is not disrupted by MMTV insertion mutation. |
