| First Author | Lupo A | Year | 1997 |
| Journal | J Biol Chem | Volume | 272 |
| Issue | 50 | Pages | 31641-7 |
| PubMed ID | 9395505 | Mgi Jnum | J:44634 |
| Mgi Id | MGI:1100657 | Doi | 10.1074/jbc.272.50.31641 |
| Citation | Lupo A, et al. (1997) Negative regulation of the mouse aldolase A gene. A cell cycle-dependent DNA binding activity functions as a silencer of gene transcription. J Biol Chem 272(50):31641-7 |
| abstractText | The expression of aldolase A L-type mRNA is increased in growth-arrested mouse NIH3T3 cells and remarkably down-regulated in actively proliferating cells. Treatment of proliferating cells with cycloheximide abolished the down-regulation of L-type mRNA expression, thus indicating that a protein factor acts as repressor in proliferating cells. Transient transfection experiments in NIH3T3 cells showed that a negative regulatory cis-element (NRE) is involved in the modulation of the transcriptional activity of the distal L promoter. The repressor, which is a protein of approximately 97 kDa, binds the murine aldolase A NRE, revealing a much more intense DNA-protein complex in proliferating NIH3T3 cells than in serum-deprived cells. Mutations in the negative regulatory cis-element showed that the GA-rich motif is required for protein binding and silencer function. We conclude that the expression of L-type mRNA is modulated by the interaction between a cell cycle-dependent DNA-binding protein and the murine aldolase A NRE. |
