| First Author | Bu X | Year | 1997 |
| Journal | J Biol Chem | Volume | 272 |
| Issue | 51 | Pages | 32613-22 |
| PubMed ID | 9405477 | Mgi Jnum | J:44732 |
| Mgi Id | MGI:1101246 | Doi | 10.1074/jbc.272.51.32613 |
| Citation | Bu X, et al. (1997) Identification of an endothelial cell-specific regulatory region in the murine endothelin-1 gene. J Biol Chem 272(51):32613-22 |
| abstractText | Endothelin-1 is a 21-amino acid peptide first characterized as a potent vasoactive compound synthesized by endothelial cells. Because of its high level cell-restricted pattern of expression, we have employed this gene as a model for investigating the DNA and protein elements that mediate endothelial cell-specific gene expression. In this study we have identified a complex positive regulatory region located at base pairs -364 to -320 in the murine endothelin-1 gene. This region consists of three functionally dependent elements, ETE-C, ETE-D, and ETE-E, which are all required for full activity. When a 43-base pair fragment containing these three elements was employed in heterologous promoter experiments, this sequence was capable of increasing transcriptional activity in an endothelial cell-specific fashion. None of the elements contains a recognized consensus sequence known to bind transcriptional regulatory proteins in higher eukaryotes; however, each element does appear to mediate protein binding. The combination of all three elements promotes binding of a protein complex that is endothelial cell-specific. This is the first evidence for an endothelial cell-specific DNA regulatory element and cognate binding proteins. |
