| First Author | de Vet EC | Year | 1998 |
| Journal | Biochem Biophys Res Commun | Volume | 242 |
| Issue | 2 | Pages | 277-81 |
| PubMed ID | 9446784 | Mgi Jnum | J:45825 |
| Mgi Id | MGI:1196164 | Doi | 10.1006/bbrc.1997.7950 |
| Citation | de Vet EC, et al. (1998) Nucleotide sequence of a cDNA clone encoding a Caenorhabditis elegans homolog of mammalian alkyl-dihydroxyacetonephosphate synthase: evolutionary switching of peroxisomal targeting signals. Biochem Biophys Res Commun 242(2):277-81 |
| abstractText | The nucleotide sequence is reported of a cDNA clone encoding a Caenorhabditis elegans homolog of guinea pig and human alkyl-dihydroxyacetonephosphate synthase. The open reading frame encodes a protein of 597 amino acids which shows extensive homology with the mammalian enzymes (52% identical and about 76% similar in the overlapping region). In contrast to the mammalian enzymes, which carry a consensus peroxisomal targeting signal type 2 in a cleavable N-terminal presequence, this Caenorhabditis elegans homolog carries a consensus peroxisomal targeting signal type 1 (CKL) at its C-terminus. Expression of this protein in an in vitro transcription/translation system yielded a 65 kDa protein. Recombinant aenorhabditis elegans alkyl-DHAP synthase expressed in the yeast Pichia pastoris was enzymatically active. |
