| First Author | Tang BL | Year | 1998 |
| Journal | Biochem Biophys Res Commun | Volume | 243 |
| Issue | 3 | Pages | 885-91 |
| PubMed ID | 9501016 | Mgi Jnum | J:46217 |
| Mgi Id | MGI:1197372 | Doi | 10.1006/bbrc.1998.8194 |
| Citation | Tang BL, et al. (1998) Hsec22c: a homolog of yeast Sec22p and mammalian rsec22a and msec22b/ERS-24. Biochem Biophys Res Commun 243(3):885-91 |
| abstractText | We have cloned a new member of a family of mammalian proteins homologous to Sec22p, a v-SNARE in Saccharomyces cerevisiae required for transport between the endoplasmic reticulum (ER) and the Golgi apparatus. The open reading frame encodes a polypeptide of 250 amino acids which is homologous to, but obviously different from, the recently reported mammalian Sec22p homologs rat sec22a, mouse sec22b, and hamster ERS-24. Northern blot analysis revealed two transcripts of about 1 and 5 kb respectively which are ubiquitously expressed. myc- epitope tagged sec22c is localized to the ER. Overexpression of the myc- tagged protein resulted in an anomalous staining pattern of SNARE molecules participating in ER-Golgi transport such as syntaxin 5 and mammalian bet1, but not the endosomal SNARE syntaxin 7. The presence of multiple forms of sec22 protein in the mammalian early secretory pathway is in-line with task specification in a highly elaborate transport machinery. |
