| First Author | Kojima K | Year | 1998 |
| Journal | Biol Pharm Bull | Volume | 21 |
| Issue | 4 | Pages | 426-8 |
| PubMed ID | 9586588 | Mgi Jnum | J:49358 |
| Mgi Id | MGI:1277399 | Doi | 10.1248/bpb.21.426 |
| Citation | Kojima K, et al. (1998) Long-term administration of sho-saiko-to increases cytochrome P-450 mRNA level in mouse liver. Biol Pharm Bull 21(4):426-8 |
| abstractText | Simplified differential display of mRNA was applied to isolate and identify genes transcriptionally regulated in mouse liver by sho-saiko-to administration. A cDNA fragment up-regulated by sho-saiko-to was isolated and characterized. cDNA sequencing and subsequent database analysis revealed that the fragment showed significant sequence similarity with mouse testosterone 16-alpha-hydroxylase (cytochrome P-450[16alpha]) cDNA. The increased level of mRNA expression of cytochrome P-450(16alpha) in association with sho-saiko-to administration suggests the molecular mechanism of the chemopreventive effect of sho-saiko-to. This result indicates the usefulness of the mRNA differential display technique to investigate the molecular mechanism of Kampo medicine. |
