| First Author | Hadjiargyrou M | Year | 1998 |
| Journal | Biochem Biophys Res Commun | Volume | 249 |
| Issue | 3 | Pages | 879-84 |
| PubMed ID | 9731230 | Mgi Jnum | J:49562 |
| Mgi Id | MGI:1277701 | Doi | 10.1006/bbrc.1998.9167 |
| Citation | Hadjiargyrou M, et al. (1998) Cloning of a novel cDNA expressed during the early stages of fracture healing. Biochem Biophys Res Commun 249(3):879-84 |
| abstractText | Using differential mRNA display (DD-PCR), a novel cDNA, FxC1 (Fracture Callus 1) was isolated from the early stages of a healing fractured femur. Utilizing 5' RACE PCR, a 598-bp full-length cDNA was obtained for FxC1 that contains an open reading frame (ORF) of 243 bp, encoding for an 80 amino acid protein. Within this ORF, a leucine zipper motif was present. In vitro transcription/translation of the full-length cDNA generated the expected 9-kDa protein. Northern analysis reveals that this gene is expressed in calluses harvested from post-fracture day 5, 7 and 10, as well as in several other tissues and bone-derived cell lines. During the differentiation of MC3T3 cells along the osteoblast lineage, FxC1 expression increases 3- to 4-fold during the production and deposition of matrix proteins, suggesting a possible role for this protein in cell differentiation. |
