| First Author | Fleet A | Year | 1998 |
| Journal | Biochem Biophys Res Commun | Volume | 250 |
| Issue | 3 | Pages | 786-90 |
| PubMed ID | 9784424 | Mgi Jnum | J:50176 |
| Mgi Id | MGI:1289997 | Doi | 10.1006/bbrc.1998.9377 |
| Citation | Fleet A, et al. (1998) Calcium buffering capacity of neuronal cell cytosol measured by flash photolysis of calcium buffer NP-EGTA. Biochem Biophys Res Commun 250(3):786-90 |
| abstractText | N1E-115 mouse neuroblastoma cells were injected with a calcium buffer/indicator solution to allow both ratiometric measurement of free calcium concentration and the release of calcium ions upon UV flash. The solution contained sulforhodamine, a marker dye used to estimate the volume injected; fluo-3, a calcium indicator, and NP-EGTA, a high affinity calcium-selective buffer that is converted by UV flash to products with negligible calcium affinity. The calcium increase recorded upon UV irradiation (Delta[Ca2+]i) was small for small injection volumes, increased with larger injection volumes, but approached a plateau at the largest injection volumes. From this relation we estimate the buffering capacity of the cytosol as 1700 ions bound per ion free. Copyright 1998 Academic Press. |
