| First Author | Domínguez A | Year | 1998 |
| Journal | Oncogene | Volume | 17 |
| Issue | 17 | Pages | 2187-93 |
| PubMed ID | 9811450 | Mgi Jnum | J:50891 |
| Mgi Id | MGI:1313013 | Doi | 10.1038/sj.onc.1202140 |
| Citation | Dominguez A, et al. (1998) hpttg, a human homologue of rat pttg, is overexpressed in hematopoietic neoplasms. Evidence for a transcriptional activation function of hPTTG. Oncogene 17(17):2187-93 |
| abstractText | We have isolated a human cDNA clone encoding a novel protein of 22 kDa that is a human counterpart of the rat oncoprotein PTTG. We show that the corresponding gene (hpttg) is overexpressed in Jurkat cells (a human T lymphoma cell line) and in samples from patients with different kinds of hematopoietic malignancies. Analysis of the sequence showed that hPTTG has an amino-terminal basic domain and a carboxyl-terminal acidic domain, and that it is a proline-rich protein with several putative SH3-binding sites. Subcellular fractionation studies show that, although hPTTG is mainly a cytosolic protein, it is partially localized in the nucleus. In addition we demonstrate that the acidic carboxyl-terminal region of hPTTG acts as a transactivation domain when fused to a heterologous DNA binding domain, both in yeast and in mammalian cells. |
