| First Author | Izquierdo M | Year | 1999 |
| Journal | EMBO J | Volume | 18 |
| Issue | 1 | Pages | 156-66 |
| PubMed ID | 9878059 | Mgi Jnum | J:51907 |
| Mgi Id | MGI:1327103 | Doi | 10.1093/emboj/18.1.156 |
| Citation | Izquierdo M, et al. (1999) Blocked negative selection of developing T cells in mice expressing the baculovirus p35 caspase inhibitor. EMBO J 18(1):156-66 |
| abstractText | Clonal deletion in the thymus by apoptosis is involved in purging the immune system of self-reactive T lymphocytes (negative selection). Cysteine proteases (caspases) belonging to the CPP32 family are activated during this process. We have produced transgenic mice expressing baculovirus p35, a broad-range caspase inhibitor. Thymocytes from p35 transgenic mice were resistant in vitro to several apoptosis-inducing agents; this resistance correlated with the inhibition of CPP32-like activity. Negative selection in vivo of thymocytes triggered by two exogenous antigens, staphylococcal enterotoxin B superantigen and an antigenic peptide in the F5 T-cell receptor transgenic model, was specifically inhibited in p35 transgenic mice. Our results provide direct evidence for caspase involvement in negative selection during thymocyte development. |
