| First Author | Takemoto N | Year | 1998 |
| Journal | Int Immunol | Volume | 10 |
| Issue | 12 | Pages | 1981-5 |
| PubMed ID | 9885919 | Mgi Jnum | J:51886 |
| Mgi Id | MGI:1327081 | Doi | 10.1093/intimm/10.12.1981 |
| Citation | Takemoto N, et al. (1998) Th2-specific DNase I-hypersensitive sites in the murine IL-13 and IL-4 intergenic region. Int Immunol 10(12):1981-5 |
| abstractText | IL-4 and IL-13 are cytokines preferentially produced by Th2 cells, and their genes are located in close proximity on human chromosome 5 and mouse chromosome 11. To identify potential regulatory elements that confer Th2-specific expression of IL-4 and IL-13 genes, we constructed a physical map of the IL-13/IL-4 locus and conducted DNase I- hypersensitive (DH) site analysis using Th clones and in vitro- differentiated effector Th cells obtained from TCR transgenic mice. Three DH sites, HSS1, HSS2 and HSS3, were identified within the intergenic region between IL-13 and IL-4 genes. HSS3 was observed both in Th1 and Th2 cells as well as CD4+ naive T cells, while HSS1 and HSS2 were detected exclusively in Th2 cells. The correlation between differentiation into Th2 subtype and the appearance of HSS1 and HSS2 suggests that these regions may play a role in subtype-specific expression of the IL-13/IL-4 locus. |
