| First Author | Matre V | Year | 1999 |
| Journal | J Neurochem | Volume | 72 |
| Issue | 1 | Pages | 40-50 |
| PubMed ID | 9886052 | Mgi Jnum | J:51652 |
| Mgi Id | MGI:1321419 | Doi | 10.1046/j.1471-4159.1999.0720040.x |
| Citation | Matre V, et al. (1999) Structural and functional organization of the gene encoding the human thyrotropin-releasing hormone receptor. J Neurochem 72(1):40-50 |
| abstractText | The thyrotropin-releasing hormone (TRH) receptor (TRHR) is widely distributed throughout the central and peripheral nervous systems. In addition to its role in controlling the synthesis and secretion of thyroid-stimulating hormone and prolactin from the anterior pituitary, TRH is believed to act as a neurotransmitter as well as a neuromodulator. We have isolated genomic lambda and P1-derived artificial chromosome clones encoding the human TRHR. The gene was found to be 35 kb with three exons and two introns. A 541-bp intron 1 (-629 to -89 relative to the translation start site) is conserved between human and mouse. A large intron 2 of 31 kb disrupts the open reading frame (starting in position +790) in the sequence encoding the supposed junction between the third intracellular loop and the putative sixth transmembrane domain. A similar intron was found in chimpanzee and sheep but not in rat and mouse. Promoter analysis of upstream regions demonstrated cell type-specific reporter activation, and sequencing of 2.5 kb of the promoter revealed putative cis-acting regulatory elements for several transcription factors that may contribute to the regulation of the TRHR gene expression. Functional analysis of potential response elements for the anterior pituitary-specific transcription factor Pit-1 revealed cell type-specific binding that was competed out with a Pit-1 response element from the GH gene promoter. |
