| First Author | Rourke IJ | Year | 1999 |
| Journal | FEBS Lett | Volume | 448 |
| Issue | 1 | Pages | 15-8 |
| PubMed ID | 10217400 | Mgi Jnum | J:54211 |
| Mgi Id | MGI:1334795 | Doi | 10.1016/s0014-5793(99)00320-8 |
| Citation | Rourke IJ, et al. (1999) Negative cooperativity between juxtaposed E-box and cAMP/TPA responsive elements in the cholecystokinin gene promoter. FEBS Lett 448(1):15-8 |
| abstractText | The promoter of the cholecystokinin (CCK) gene possesses evolutionary conserved juxtaposed E-box and cAMP/TPA responsive elements (CRE/TRE). We have examined the functional interaction of these two sites. As previously noted, c-Jun/c-Fos heterodimers greatly increase promoter activity through association with the CRE/TRE. Mutation of the E-box enhanced the activation by c-Jun/c-Fos, as well as stimulation by forskolin and bFGF, that acts through the CRE/TRE site. Moreover, c-Jun/c-Fos stimulation was inhibited by co-expression of c-Myc and Max. The results indicate that factors associating with the E-box exhibit a negative cooperative effect on the activation via the CRE/TRE element. We propose that this mechanism plays a significant role in CCK gene transcription and other genes with juxtaposed E-box and CRE/TRE. |
