| First Author | Chin LS | Year | 1999 |
| Journal | Brain Res Mol Brain Res | Volume | 67 |
| Issue | 2 | Pages | 239-46 |
| PubMed ID | 10216222 | Mgi Jnum | J:54875 |
| Mgi Id | MGI:1336406 | Doi | 10.1016/s0169-328x(99)00066-2 |
| Citation | Chin LS, et al. (1999) Neuron-specific and developmental regulation of the synapsin II gene expression in transgenic mice. Brain Res Mol Brain Res 67(2):239-46 |
| abstractText | Synapsin II, a major phosphoprotein of synaptic vesicles, is believed to function in neurotransmitter release as well as in synapse formation. The expression of the synapsin II gene is neuron-specific, and correlates temporally with synaptogenesis. To understand the mechanisms by which the expression of the synapsin II gene is regulated in vivo, we generated transgenic mice carrying a 5.1-kb 5'-flanking sequence of the murine synapsin II gene fused to the firefly luciferase reporter gene. The synapsin II-luciferase transgene is specifically expressed in neural tissues, such as brain and spinal cord, but not in non-neural tissues. Throughout the brain, the expression of the transgene is widely distributed, and restricted only to neuronal cells. Moreover, the expression of the transgene is developmentally regulated, with a temporal profile similar to that of endogenous synapsin II expression. These results indicate that the 5.1-kb flanking sequence of the murine synapsin II gene contains cis-regulatory elements that are required for directing neuron-specific and synaptogenesis-regulated expression in vivo. Copyright 1999 Elsevier Science B.V. |
