| First Author | Rocca B | Year | 1999 |
| Journal | J Clin Invest | Volume | 103 |
| Issue | 10 | Pages | 1469-77 |
| PubMed ID | 10330429 | Mgi Jnum | J:55108 |
| Mgi Id | MGI:1337239 | Doi | 10.1172/JCI6400 |
| Citation | Rocca B, et al. (1999) Distinct roles of prostaglandin H synthases 1 and 2 in T-cell development. J Clin Invest 103(10):1469-77 |
| abstractText | Prostaglandin G and H synthases, or cyclooxygenases (COXs), catalyze the formation of prostaglandins (PGs). Whereas COX-1 is diffusely expressed in lymphoid cells in embryonic day 15.5 thymus, COX-2 expression is sparse, apparently limited to stromal cells. By contrast, COX-2 is predominant in a subset of medullary stromal cells in three- to five-week-old mice. The isozymes also differ in their contributions to lymphocyte development. Thus, experiments with selective COX-1 inhibitors in thymic lobes from normal and recombinase-activating gene-1 knockout mice support a role for this isoform in the transition from CD4(-)CD8(-) double-negative (DN) to CD4(+)CD8(+) double-positive (DP). Concordant data were obtained in COX-1 knockouts. Pharmacological inhibition and genetic deletion of COX-2, by contrast, support its role during early thymocyte proliferation and differentiation and, later, during maturation of the CD4 helper T-cell lineage. PGE2, but not other PGs, can rescue the effects of inhibition of either isoform, although it acts through distinct EP receptor subtypes. COX-dependent PG generation may represent a mechanism of thymic stromal support for T-cell development. |
