| First Author | Atanasova E | Year | 1999 |
| Journal | J Biol Chem | Volume | 274 |
| Issue | 30 | Pages | 21078-84 |
| PubMed ID | 10409660 | Mgi Jnum | J:56406 |
| Mgi Id | MGI:1340926 | Doi | 10.1074/jbc.274.30.21078 |
| Citation | Atanasova E, et al. (1999) Novel messenger RNA and alternative promoter for murine acetylcholinesterase. J Biol Chem 274(30):21078-84 |
| abstractText | A portion of the 5'-flanking region of murine acetylcholinesterase was cloned from genomic DNA by 5'-rapid amplification of genomic ends, identified in a mouse genomic library, and sequenced. Multiple potential binding sites for universal and tissue-specific transcription factors were suggestive of a promoter region within this DNA sequence. Potential promoter activity was confirmed by coupling the new sequence to the open reading frame of a luciferase reporter gene in transient expression experiments with nerve and muscle cells. 5'-Rapid amplification of cDNA ends with templates from multiple sources revealed a novel transcription start site (at position -626, relative to translation start), located 32 bases downstream from a TATAA sequence. This start site appeared to mark a novel exon (1a) comprising 291 base pairs between positions -335 and -626, relative to the translation start. Supporting this conclusion, polymerase chain reactions with cDNA from mouse brain, heart, and other tissues, consistently amplified a transcript containing the exon 1a sequence fused to the invariant sequence beginning at position -22 in exon 2, but lacking exon 1. Northern blot analyses confirmed the in vivo expression of exon 1a-containing transcripts, especially in heart, brain, liver, and kidney. These results indicate that the murine acetylcholinesterase gene has a functioning alternative promoter that may influence expression of acetylcholinesterase in certain tissues. |
