| First Author | Song F | Year | 2002 |
| Journal | J Autoimmun | Volume | 18 |
| Issue | 1 | Pages | 27-37 |
| PubMed ID | 11869044 | Mgi Jnum | J:75590 |
| Mgi Id | MGI:2177099 | Doi | 10.1006/jaut.2001.0567 |
| Citation | Song F, et al. (2002) Differences Between Two Strains of Myelin Basic Protein (MBP) TCR Transgenic Mice: Implications for Tolerance Induction. J Autoimmun 18(1):27-37 |
| abstractText | Experimental autoimmune encephalomyelitis (EAE) is mediated by CD4(+) T cells which preferentially use the Vbeta8.2 TCR in response to myelin basic protein (MBP). Two strains of Tg mice (Valpha2.3/Vbeta8.2 and Valpha4/Vbeta8.2) have T cell receptors that recognize the NAc1-11 immunodominant epitope of MBP. We previously reported that oral administration of MBP protects both Valpha2.3/Vbeta8.2 and Valpha4/Vbeta8.2 mice from EAE; however, tolerance induction differs between strains and is dependent on the timing of oral antigen. Here we analyze the peripheral and gut-associated lymphoid tissue (GALT) environments of the two strains of Tg mice. Tg cells in the Peyer's patch (PP) but not the spleen of Valpha2.3/Vbeta8.2 mice demonstrate increased CD69 and decreased CD45RB relative to Valpha4/Vbeta8.2 mice. High levels of Th1 and Th2 cytokines, proliferative activity and CC chemokines (MCP-1) are observed in the periphery and GALT of Valpha2.3/Vbeta8.2 Tg mice. In contrast, more non-Tg CD4(+) cells are seen in the PP of Valpha4/Vbeta8.2 mice. These studies suggest that activated Tg T cells and fewer potential regulatory cells in the PP of Valpha2.3/Vbeta8.2 Tg mice may influence oral tolerance. |
