| First Author | Grandemange S | Year | 2001 |
| Journal | BMC Mol Biol | Volume | 2 |
| Pages | 14 | PubMed ID | 11747469 |
| Mgi Jnum | J:73585 | Mgi Id | MGI:2155924 |
| Doi | 10.1186/1471-2199-2-14 | Citation | Grandemange S, et al. (2001) A human RNA polymerase II subunit is encoded by a recently generated multigene family. BMC Mol Biol 2(1):14 |
| abstractText | BACKGROUND: The sequences encoding the yeast RNA polymerase II (RPB) subunits are single copy genes. RESULTS: While those characterized so far for the human (h) RPB are also unique, we show that hRPB subunit 11 (hRPB11) is encoded by a multigene family, mapping on chromosome 7 at loci p12, q11.23 and q22. We focused on two members of this family, hRPB11a and hRPB11b: the first encodes subunit hRPB11a, which represents the major RPB11 component of the mammalian RPB complex; the second generates polypeptides hRPB11balpha and hRPB11bbeta through differential splicing of its transcript and shares homologies with components of the hPMS2L multigene family related to genes involved in mismatch-repair functions (MMR). Both hRPB11a and b genes are transcribed in all human tissues tested. Using an inter-species complementation assay, we show that only hRPB11balpha is functional in yeast. In marked contrast, we found that the unique murine homolog of RPB11 gene maps on chromosome 5 (band G), and encodes a single polypeptide which is identical to subunit hRPB11a. CONCLUSIONS: The type hRPB11b gene appears to result from recent genomic recombination events in the evolution of primates, involving sequence elements related to the MMR apparatus. |
