| First Author | Manzano M | Year | 2002 |
| Journal | J Nutr | Volume | 132 |
| Issue | 9 | Pages | 2757-62 |
| PubMed ID | 12221241 | Mgi Jnum | J:78937 |
| Mgi Id | MGI:2386503 | Doi | 10.1093/jn/132.9.2757 |
| Citation | Manzano M, et al. (2002) Absolute Counts and Distribution of Lymphocyte Subsets in Small Intestine of BALB/c Mice Change during Weaning. J Nutr 132(9):2757-62 |
| abstractText | The gut immune system is an essential part of the barrier function of the gut. At weaning, major changes can be expected in the number and subset composition of lymphocytes in the small intestine since the gut is exposed to a wide variety of food and microbial antigens, especially when human milk is gradually replaced by weaning foods. The purpose of this study was to evaluate the changes in small intestine lymphocyte subsets in mice during weaning. BALB/c male mice at weaning (3 wk old) were fed a nonpurified diet for 18 d and were killed at different times (0, 4, 7, 12 and 18 d). Lymphocyte populations from lamina propria (LPL), Peyer's patches (PPL) and intestinal epithelium (IEL) were isolated. The expression of different antigens (CD3, CD4, CD8alpha, CD8beta, CD22 and CD45R) in those lymphocyte populations was analyzed by flow cytometry. The percentages of cells expressing T-cell antigens, such as CD3, were significantly higher in LPL during weaning compared to d 0. The percentages of cells expressing CD8alpha and CD8beta increased in both IEL and LPL. However, the percentage of CD4+ cells tended (P = 0.07) to decrease in IEL and to increase in LPL. The percentages of cells expressing B-cell antigens, such as CD22 or CD45R in PPL increased. Changes in the specific phenotypes of intestinal lymphocyte populations at weaning are apparently related to the maturation of the intestinal immune system during early life. Thus, B cells increase in PPL and T cell increase in IEL and LPL. |
