| First Author | Kos M | Year | 2002 |
| Journal | J Biol Chem | Volume | 277 |
| Issue | 40 | Pages | 37131-8 |
| PubMed ID | 12147702 | Mgi Jnum | J:79348 |
| Mgi Id | MGI:2387887 | Doi | 10.1074/jbc.M206325200 |
| Citation | Kos M, et al. (2002) Upstream Open Reading Frames Regulate the Translation of the Multiple mRNA Variants of the Estrogen Receptor alpha. J Biol Chem 277(40):37131-8 |
| abstractText | It is by now well established that the estrogen receptor alpha (ERalpha) is transcribed from multiple promoters. One direct consequence of multiple promoters is the generation of mRNA variants with different 5'-untranslated regions (5'-UTRs). However, the potential roles of these individual mRNA variants are not known. All 5'-UTRs of ERalpha contain between one and six upstream open reading frames. In this study the effect of the 5'-UTRs of major human and mouse ERalpha mRNA variants on translation was evaluated. Some of the 5'-UTRs were found to strongly inhibit translation of the downstream open reading frame. Mutation of the upstream AUG codons partially or completely restored translation efficiency. A toeprinting analysis and assessment of the contribution of each AUG codon to the inhibitory effect on translation showed that leaky scanning and reinitiation occurs with these mRNAs. In conclusion, the upstream open reading frames in the 5'-UTRs of ERalpha mRNAs have the potential to regulate estrogen receptor alpha expression. |
