| First Author | Cutler CP | Year | 2002 |
| Journal | Biochim Biophys Acta | Volume | 1566 |
| Issue | 1-2 | Pages | 92-103 |
| PubMed ID | 12421541 | Mgi Jnum | J:80125 |
| Mgi Id | MGI:2429828 | Doi | 10.1016/s0005-2736(02)00596-5 |
| Citation | Cutler CP, et al. (2002) Two isoforms of the Na(+)/K(+)/2Cl(-) cotransporter are expressed in the European eel (Anguilla anguilla). Biochim Biophys Acta 1566(1-2):92-103 |
| abstractText | Two cDNA isoforms of the NKCC1 secretory cotransporter have been isolated from the European eel. The NKCC1a isoform exhibited mRNA expression in a wide range of tissues in a similar fashion to mammals, whereas NKCC1b was expressed primarily in the brain. The effect of freshwater (FW) to seawater (SW) transfer on NKCC1a expression was dependent on the developmental stage. In non-migratory yellow eels, NKCC1a mRNA expression in the gill was transiently up-regulated 4.3-fold after 2 days but also subsequently by 2.5-6-fold 3 weeks after SW transfer. Gill NKCC1a expression was localised mainly in branchial chloride cells of SW acclimated yellow eels. In contrast to yellow eels, NKCC1a mRNA abundance was not significantly different following SW acclimation in silver eel gill. NKCC1a mRNA abundance decreased in the kidney following SW acclimation and this may correlate with lower tubular ion/fluid secretion and urine flow rates in SW teleosts. Kidney NKCC1a mRNA expression in silver eels was also significantly lower than in yellow eels, suggesting some pre-acclimation of mRNA levels. NKCC1a mRNA was expressed at similar low levels in the middle intestine of FW- and SW-acclimated yellow or silver eels, suggesting the presence of an ion secretory mechanism in this gut segment. |
