| First Author | Harrison M | Year | 2002 |
| Journal | Biochim Biophys Acta | Volume | 1579 |
| Issue | 2-3 | Pages | 164-72 |
| PubMed ID | 12427551 | Mgi Jnum | J:80334 |
| Mgi Id | MGI:2445674 | Doi | 10.1016/s0167-4781(02)00544-4 |
| Citation | Harrison M, et al. (2002) Genetics and differential expression of NADH:ubiquinone oxidoreductase B8 subunit in brains of genetic strains of mice differing in voluntary alcohol consumption. Biochim Biophys Acta 1579(2-3):164-72 |
| abstractText | Inbred strains of mice remain a valuable resource for genetic dissection of complex traits including responses to drugs and chemicals, particularly alcohol. As a novel source of candidate genes for further analysis, we have used mRNA differential displays to identify genes with differential expression in the brains of ethanol-preferring (C57BL/6J) vs. ethanol-avoiding (A/J, BALB/c, and DBA/2J) strains, with and without ethanol i.p. treatments (4 g/kg). We report on one such gene, NADH:ubiquinone oxidoreductase B8 subunit, that has a higher expression in the C57BL/6J. Further, its expression also increases following ethanol treatment as compared to the three alcohol-avoiding strains. This regulatory feature follows three single nucleotide polymorphisms (SNPs) in the promoter region across the four strains studied. The four strains represent only two haplotypes, one C57BL/6J-specific and the other found in the three alcohol-avoiding strains. Interestingly, one of the observed SNPs (-687 A/G) is located in the putative TFIID binding site with potential to regulate the expression of this gene and contribute to genotype-specific alcohol responses and effects involving reactive oxygen species (ROS). |
