| First Author | Xirodimas DP | Year | 2002 |
| Journal | FEBS Lett | Volume | 528 |
| Issue | 1-3 | Pages | 207-11 |
| PubMed ID | 12297306 | Mgi Jnum | J:79230 |
| Mgi Id | MGI:2387531 | Doi | 10.1016/s0014-5793(02)03310-0 |
| Citation | Xirodimas DP, et al. (2002) P14ARF promotes accumulation of SUMO-1 conjugated (H)Mdm2. FEBS Lett 528(1-3):207-11 |
| abstractText | p14ARF tumour suppressor stabilises and activates p53 by directly interacting with (H)Mdm2 [(human) murine double minute 2 homologue] and inhibiting its E3 ubiquitin ligase activity. Here we demonstrate that p14ARF promotes accumulation of (H)Mdm2 conjugated to the small ubiquitin-like protein SUMO-1. Mutational analysis demonstrated that the N-terminus of Mdm2 is a target for p14ARF-mediated SUMO conjugation. SUMO modification requires residues 2-14 in p14ARF that interact with (H)Mdm2 and residues 82-101 in exon 2 involved in nucleolar localisation of p14ARF. These data suggest a novel role for p14ARF as a regulator of activity of (H)Mdm2, which could be related to its tumour suppressing activities. |
