| First Author | Cool SM | Year | 2002 |
| Journal | Histochem J | Volume | 34 |
| Issue | 6-7 | Pages | 291-7 |
| PubMed ID | 12769260 | Mgi Jnum | J:84134 |
| Mgi Id | MGI:2665256 | Doi | 10.1023/a:1023326524562 |
| Citation | Cool SM, et al. (2002) Temporal and spatial expression of fibroblast growth factor receptor 4 isoforms in murine tissues. Histochem J 34(6-7):291-7 |
| abstractText | Fibroblast growth factor receptors (FGFRs) undergo highly regulated spatial and temporal changes of expression during development. This study describes the use of quantitative reverse transcriptase-polymerase chain reaction and immunochemistry to assess the changes in expression of FGFR4 as compared to its FGFR4-17a and -17b isoforms in mouse tissues, from early embryogenesis through to adulthood. Compared to FGFR4, the expression of the isoforms is more restricted at all developmental stages tested. The reverse transcriptase-polymerase chain reaction demonstrated that FGFR4 is expressed in more tissue types than either of its isoforms: it was found predominantly in lung, liver, brain, skeletal muscle and kidney, whereas the FGFR4-17a form was detected in lung and skeletal muscle, and the FGFR4-17b form only in lung, liver, skeletal muscle and kidney. Immunohistochemistry confirmed strong FGFR4-17b expression in the postnatal lung. When combined, the results suggest that FGFR4 variants play important roles particularly in lung and skeletal muscle development. |
