| First Author | Sousa-Nunes R | Year | 2003 |
| Journal | Genome Res | Volume | 13 |
| Issue | 12 | Pages | 2609-20 |
| PubMed ID | 14613977 | Mgi Jnum | J:85124 |
| Mgi Id | MGI:2672891 | Doi | 10.1101/gr.1362303 |
| Citation | Sousa-Nunes R, et al. (2003) Characterizing embryonic gene expression patterns in the mouse using nonredundant sequence-based selection. Genome Res 13(12):2609-20 |
| abstractText | This article investigates the expression patterns of 160 genes that are expressed during early mouse development. The cDNAs were isolated from 7.5 d postcoitum (dpc) endoderm, a region that comprises visceral endoderm (VE), definitive endoderm, and the node-tissues that are required for the initial steps of axial specification and tissue patterning in the mouse. To avoid examining the same gene more than once, and to exclude potentially ubiquitously expressed housekeeping genes, cDNA sequence was derived from 1978 clones of the Endoderm library. These yielded 1440 distinct cDNAs, of which 123 proved to be novel in the mouse. In situ hybridization analysis was carried out on 160 of the cDNAs, and of these, 29 (18%) proved to have restricted expression patterns. |
