| First Author | Forné I | Year | 2003 |
| Journal | J Biol Chem | Volume | 278 |
| Issue | 50 | Pages | 50641-4 |
| PubMed ID | 14523012 | Mgi Jnum | J:87171 |
| Mgi Id | MGI:2683820 | Doi | 10.1074/jbc.M308531200 |
| Citation | Forne I, et al. (2003) Identification of the autoantigen HB as the barrier-to-autointegration factor. J Biol Chem 278(50):50641-4 |
| abstractText | The HB autoantigen, a 10-kDa DNA-binding protein recognized by autoantibodies only when bound to DNA, was identified by two-dimensional electrophoresis. Silver-stained protein spots corresponding to the antigen were excised from two-dimensional electrophoresis gels, digested with trypsin, and analyzed by matrix-assisted laser desorption/ionization-reflectron time of flight and nano-electrospray ionization-ion trap/mass spectrometry. Data base search identified the HB antigen as the barrier-to-autointegration factor, a cellular protein implicated in the cellular cycle that blocks autointegration and promotes intermolecular integration of retrovirus such as the Moloney murine leukemia and the human immunodeficiency type 1 virus. The physicochemical characteristics described for these proteins, their ability to bind double-stranded DNA but not single-stranded DNA, and their nuclear localization confirm that HB and barrier-to-autointegration factor are the same protein. |
