| First Author | Hachiya NS | Year | 2004 |
| Journal | Biochem Biophys Res Commun | Volume | 313 |
| Issue | 3 | Pages | 818-23 |
| PubMed ID | 14697265 | Mgi Jnum | J:87458 |
| Mgi Id | MGI:2687152 | Doi | 10.1016/j.bbrc.2003.11.167 |
| Citation | Hachiya NS, et al. (2004) Microtubules-associated intracellular localization of the NH2-terminal cellular prion protein fragment. Biochem Biophys Res Commun 313(3):818-23 |
| abstractText | By utilizing double-labeled fluorescent cellular prion protein (PrPC), we revealed that the NH2-terminal and COOH-terminal PrPC fragments exhibit distinct distribution patterns in mouse neuroblastoma neuro2a (N2a) cells and HpL3-4, a hippocampal cell line established from prnp gene-ablated mice [Nature 400 (1999) 225]. Of note, the NH2-terminal PrPC fragment, which predominantly localized in the intracellular compartments, congregated in the cytosol after the treatment with a microtubule depolymerizer (nocodazole). Truncated PrPC with the amino acid residues 1-121, 1-111, and 1-91 in mouse (Mo) PrP exhibited a proper distribution profile, whereas those with amino acid residues 1-52 and 1-33 did not. These data indicate the microtubules-associated intracellular localization of the NH2-terminal PrPC fragment containing at least the 1-91 amino acid residues. |
