| First Author | Elluru RG | Year | 2004 |
| Journal | Arch Otolaryngol Head Neck Surg | Volume | 130 |
| Issue | 6 | Pages | 732-6 |
| PubMed ID | 15210554 | Mgi Jnum | J:91316 |
| Mgi Id | MGI:3046476 | Doi | 10.1001/archotol.130.6.732 |
| Citation | Elluru RG, et al. (2004) Potential role of Sox9 in patterning tracheal cartilage ring formation in an embryonic mouse model. Arch Otolaryngol Head Neck Surg 130(6):732-6 |
| abstractText | OBJECTIVE: To identify genes expressed early in the formation of the mouse trachea that control patterning of tracheal cartilaginous rings. DESIGN: The mouse larynx and trachea begin as an outpouching from the ventral foregut endoderm at embryonic day (E) 9. Digoxigenin-labeled RNA probes to putative tracheal patterning genes were generated by in vitro transcription. Embryos ranging in age from E9 to E16 were then subjected to whole-mount in situ hybridization using these labeled RNA probes. The RNA probes were then localized using antidigoxigenin antibodies tagged with a reporter molecule. In this manner, the 3-dimensional spatial and temporal expression of putative tracheal patterning genes was examined.Subjects F/VBN mice. RESULTS: In the developing mouse trachea, the expression of Sox9 messenger RNA preceded cartilage ring formation. Sox9 was expressed as 2 distinct longitudinal stripes along the posterolateral aspect of the trachea as early as E9, when the developing trachea is first identified. Collagen 2A1, a cartilage-specific protein, was subsequently expressed in the same longitudinal pattern as Sox9, consistent with the early commitment of Sox9-expressing cells to the cartilage program. As cartilage rings formed, Sox9 and collagen 2A1 was expressed over the lateral and anterior aspects of the trachea. CONCLUSIONS: We have developed a system to study the early expression of genes that may pattern the formation of the trachea. We have identified a gene (Sox9) with a known role in chondrocyte differentiation that is expressed in a highly specific temporal and spatial pattern in the developing upper respiratory tract. |
