| First Author | Yu K | Year | 2004 |
| Journal | J Biol Chem | Volume | 279 |
| Issue | 8 | Pages | 6496-500 |
| PubMed ID | 14645244 | Mgi Jnum | J:88965 |
| Mgi Id | MGI:3037516 | Doi | 10.1074/jbc.M311616200 |
| Citation | Yu K, et al. (2004) DNA substrate length and surrounding sequence affect the activation-induced deaminase activity at cytidine. J Biol Chem 279(8):6496-500 |
| abstractText | Activation-induced deaminase (AID) is required for both immunoglobulin class switch recombination and somatic hypermutation. AID is known to deaminate cytidines in single-stranded DNA, but the relationship of this step to the class switch or somatic hypermutation processes is not entirely clear. We have studied the activity of a recombinant form of the mouse AID protein that was purified from a baculovirus expression system. We find that the length of the single-stranded DNA target is critical to the action of AID at the Cs positioned anywhere along the length of the DNA. The DNA sequence surrounding a given C influences AID deamination efficiency. AID preferentially deaminates Cs in the WRC motif, and additionally has a small but consistent preference for purine at the position after the WRC, thereby favoring WRCr (the lowercase r corresponds to the smaller impact on activity). |
