| First Author | Xu TR | Year | 2004 |
| Journal | FEBS Lett | Volume | 570 |
| Issue | 1-3 | Pages | 20-4 |
| PubMed ID | 15251432 | Mgi Jnum | J:91383 |
| Mgi Id | MGI:3046627 | Doi | 10.1016/j.febslet.2004.05.080 |
| Citation | Xu TR, et al. (2004) Phorbol ester-induced translocation of PKC epsilon to the nucleus in fibroblasts: identification of nuclear PKC epsilon-associating proteins. FEBS Lett 570(1-3):20-4 |
| abstractText | We show that phorbol ester treatment of NIH 3T3 fibroblasts induces rapid translocation of PKC from a perinuclear site to the nucleus, extending findings in PC12 and NG108-15 cells and in myocytes. We have immunoprecipitated the PKC from nuclei isolated from phorbol ester-treated fibroblasts and identified six proteins which associate with nuclear PKC. These have been characterised as matrin 3, transferrin, Rac GTPase activating protein 1, vimentin, beta-actin and annexin II by MALDI-TOF-MS. We have confirmed that these proteins associate with PKC by gel overlay and/or dot blotting assays. The role of these PKC-associating proteins in the nucleus and their interaction with PKC are considered. |
