| First Author | Tanaka S | Year | 2005 |
| Journal | Eur J Immunol | Volume | 35 |
| Issue | 2 | Pages | 460-8 |
| PubMed ID | 15668925 | Mgi Jnum | J:95615 |
| Mgi Id | MGI:3526630 | Doi | 10.1002/eji.200425622 |
| Citation | Tanaka S, et al. (2005) Ca(2+) influx-mediated histamine synthesis and IL-6 release in mast cells activated by monomeric IgE. Eur J Immunol 35(2):460-8 |
| abstractText | We previously demonstrated that histamine synthesis is drastically induced upon sensitization with an anti-DNP IgE clone, SPE-7, in IL-3-dependent mouse bone marrow derived mast cells (BMMC). We found that Ca(2+) mobilization induced by SPE-7 exhibited a similar profile to the capacitative Ca(2+) entry evoked by thapsigargin. Potentials for activation of mast cells were found to vary between different IgE clones, and a monovalent hapten, DNP-lysine, suppressed the activation induced by SPE-7. Ca(2+) mobilization induced by SPE-7 was suppressed potently by the specific store-operated Ca(2+) channel inhibitor, SK&F 96365, but not at all by Ca(2+) channel inhibitors with more broad spectrum, La(3+) and Gd(3+), whereas the Ca(2+) mobilization induced by Ag stimulation was suppressed by these inhibitors. Ca(2+) mobilization was also induced by SPE-7 in in vitro differentiated mast cells, although the increases in histamine synthesis and IL-6 release were smaller than those in BMMC. These results suggest that Ca(2+) influx operated by a distinct mechanism from that in Ag stimulation is essential for increased histamine synthesis and IL-6 release in mast cells. |
