| First Author | Brossard C | Year | 2005 |
| Journal | Eur J Immunol | Volume | 35 |
| Issue | 6 | Pages | 1741-53 |
| PubMed ID | 15909310 | Mgi Jnum | J:99188 |
| Mgi Id | MGI:3581458 | Doi | 10.1002/eji.200425857 |
| Citation | Brossard C, et al. (2005) Multifocal structure of the T cell - dendritic cell synapse. Eur J Immunol 35(6):1741-1753 |
| abstractText | The structure of immunological synapses formed between murine naive T cells and mature dendritic cells has been subjected to a quantitative analysis. Immunofluorescence images of synapses formed in the absence of antigen show a diffuse synaptic accumulation of CD3 and LFA-1. In electron microscopy, these antigen-free synapses present a number of tight appositions (cleft size approximately 15 nm), all along the synapse. These tight appositions cover a significantly larger surface fraction of antigen-dependent synapses. In immunofluorescence, antigen-dependent synapses show multiple patches of CD3 and LFA-1 with a variable overlap. A similar distribution is observed for PKCtheta and talin. A concentric organization characteristic of prototypical synapses is rarely observed, even when dendritic cells are paralyzed by cytoskeletal poisons. In T-DC synapses, the interaction surface is composed of several tens of submicronic contact spots, with no large-scale segregation of CD3 and LFA-1. As a comparison, in T-B synapses, a central cluster of CD3 is frequently observed by immunofluorescence, and electron microscopy reveals a central tight apposition. Our data show that it is inappropriate to consider the concentric structure as a 'mature synapse' and multifocal structures as immature. |
