| First Author | Moon HE | Year | 2005 |
| Journal | FEBS Lett | Volume | 579 |
| Issue | 17 | Pages | 3797-801 |
| PubMed ID | 15978586 | Mgi Jnum | J:99779 |
| Mgi Id | MGI:3583831 | Doi | 10.1016/j.febslet.2005.05.072 |
| Citation | Moon HE, et al. (2005) Negative regulation of hypoxia inducible factor-1alpha by necdin. FEBS Lett 579(17):3797-801 |
| abstractText | Hypoxia-inducible factor 1 (HIF-1) is a master transcription factor that mediates cellular and systemic homeostatic responses to reduce O2 availability, such as erythropoiesis, angiogenesis, and glycolysis. Using the yeast two-hybrid screening system, we found that the oxygen dependent degradation (ODD) domain of HIF-1alpha interacts with necdin, a growth suppressor. The interaction of necdin with HIF-1alpha was confirmed using coimmunoprecipitation with the overexpressed HIF-1alpha. Biological effect of necdin on HIF-1alpha showed that necdin reduces the transcriptional activity of HIF-1 under hypoxia. Moreover, necdin decreased the level of the HIF-1alpha protein, but not that of mRNA, implying a possibility of necdin-mediated HIF-1alpha degradation. Furthermore, necdin has an anti-angiogenic activity in the tube formation assay and CAM assay, which might be due to the downregulation of HIF-1alpha. Collectively, these results suggest that necdin can be a novel negative regulator of HIF-1alpha stability via the direct interaction. |
