| First Author | Kim JH | Year | 2005 |
| Journal | J Immunol | Volume | 175 |
| Issue | 1 | Pages | 147-54 |
| PubMed ID | 15972641 | Mgi Jnum | J:100613 |
| Mgi Id | MGI:3588934 | Doi | 10.4049/jimmunol.175.1.147 |
| Citation | Kim JH, et al. (2005) Independent trafficking of Ig-alpha/Ig-beta and mu-heavy chain is facilitated by dissociation of the B cell antigen receptor complex. J Immunol 175(1):147-54 |
| abstractText | The BCR relays extracellular signals and internalizes Ag for processing and presentation. We have previously demonstrated that ligation of the BCR destabilizes Ig-alpha/Ig-beta (Ig-alphabeta) from mu-H chain (mum). In this study we report that receptor destabilization represents a physical separation of mum from Ig-alphabeta. Sucrose gradient fractionation localized Ig-alphabeta to G(M1)-containing lipid microdomains in the absence of mum. Confocal and electron microscopy studies revealed the colocalization of unsheathed mum with clathrin-coated vesicles. Furthermore, mum failed to associate with clathrin-coated vesicles when receptor destabilization was inhibited, suggesting that unsheathing of mum is required for clathrin-mediated endocytosis. In summary, we found that Ag stimulation physically separates Ig-alphabeta from mum, facilitating concomitant signal transduction and Ag delivery to the endocytic compartment. |
