| First Author | Qi Y | Year | 2005 |
| Journal | Eur J Neurosci | Volume | 22 |
| Issue | 6 | Pages | 1277-86 |
| PubMed ID | 16190883 | Mgi Jnum | J:101560 |
| Mgi Id | MGI:3604263 | Doi | 10.1111/j.1460-9568.2005.04339.x |
| Citation | Qi Y, et al. (2005) PQBP-1 is expressed predominantly in the central nervous system during development. Eur J Neurosci 22(6):1277-86 |
| abstractText | Mutations of PQBP-1 (polyglutamine binding protein-1) have been shown recently to cause human mental retardation accompanied by microcephaly at a high frequency. As a first step towards understanding the molecular basis of this developmental anomaly, we analysed developmental expression of PQBP-1 by in situ hybridization, immunohistochemistry and Western blot analysis. Although it had been shown by Northern blot analysis that PQBP-1 mRNA is expressed in multiple organs in adult mice, our present results revealed that PQBP-1 mRNA and protein are dominantly expressed in the central nervous system (CNS) in embryos and in newborn mice. The mean expression level of PQBP-1 reaches a peak around birth and is down-regulated in adulthood. Furthermore, the expression pattern in the CNS changes remarkably following birth. PQBP-1 mRNA in the cerebral cortex is high in embryos but it rapidly decreases after birth. PQBP-1 mRNA increases in external and internal granular cell layers of the cerebellum from postnatal day 1 (P1) to P5. In addition, expression in the subventricular zone, where neurogenesis occurs, was high from P5 to adulthood. Collectively, these findings suggest that PQBP-1 might be involved in neuronal proliferation and/or maturation. These ideas may be relevant to the insufficient growth of brain structure reported in PQBP-1-linked human mental retardation. |
