| First Author | Manninen A | Year | 2005 |
| Journal | Mol Cell Biol | Volume | 25 |
| Issue | 22 | Pages | 10087-96 |
| PubMed ID | 16260622 | Mgi Jnum | J:102356 |
| Mgi Id | MGI:3607406 | Doi | 10.1128/MCB.25.22.10087-10096.2005 |
| Citation | Manninen A, et al. (2005) Caveolin-1 is not essential for biosynthetic apical membrane transport. Mol Cell Biol 25(22):10087-96 |
| abstractText | Caveolin-1 has been implicated in apical transport of glycosylphosphatidylinositol (GPI)-anchored proteins and influenza virus hemagglutinin (HA). Here we have studied the role of caveolin-1 in apical membrane transport by generating caveolin-1-deficient Madin-Darby canine kidney (MDCK) cells using retrovirus-mediated RNA interference. The caveolin-1 knockdown (cav1-KD) MDCK cells were devoid of caveolae. In addition, caveolin-2 was retained in the Golgi apparatus in cav1-KD MDCK cells. However, we found no significant alterations in the apical transport kinetics of GPI-anchored proteins or HA upon depletion of caveolin-1. Similar results were obtained using embryonic fibroblasts from caveolin-1-knockout mice. Thus, we conclude that caveolin-1 does not play a major role in lipid raft-mediated biosynthetic membrane trafficking. |
