| First Author | Kawagishi R | Year | 2005 |
| Journal | Eur J Obstet Gynecol Reprod Biol | Volume | 121 |
| Issue | 1 | Pages | 71-6 |
| PubMed ID | 15989986 | Mgi Jnum | J:103258 |
| Mgi Id | MGI:3609008 | Doi | 10.1016/j.ejogrb.2004.12.019 |
| Citation | Kawagishi R, et al. (2005) Expression of nectin-2 in mouse granulosa cells. Eur J Obstet Gynecol Reprod Biol 121(1):71-6 |
| abstractText | OBJECTIVE: The development and maturation of the ovarian follicles are characterized by structural changes that require components involved in cell-cell adhesion systems. Recently a novel group of cell adhesion molecules named nectins has been identified. The present study examined expression and cell-specific localization of nectins during mouse follicular development. STUDY DESIGN: Expression of nectins in mouse ovary was investigated by immnuoblot analysis and immunohistochemistry. More precise localization was determined by electron microscopy. RESULTS: Immunoblot analysis revealed expression of nectin-2 and nectin-3 but not nectin-1 in ovarian granulosa cells. Immunohistochemistry demonstrated expression of nectin-2 at cell-cell adhesion sites of granulosa cell layer in the primary and preantral follicles. Especially, intense immunoreactivity of nectin-2 accumulated around the zona pellucida. In antral follicles, the intensity of nectin-2 expression on granulosa cells was decreased. By electron microscopy nectin-2 was detected not only on thin extensions of granulosa cells penetrating the zona pellucida, but also on the attachment sites between thin extensions of granulosa cells and oocyte surface. CONCLUSION: The restricted expression of nectin-2 in the granulosa cells of primary and preantral follicles might reflect some of the molecular changes in cell-cell adhesion during early follicular development. |
