| First Author | Yao G | Year | 2006 |
| Journal | Int Immunol | Volume | 18 |
| Issue | 1 | Pages | 19-29 |
| PubMed ID | 16361318 | Mgi Jnum | J:104173 |
| Mgi Id | MGI:3611418 | Doi | 10.1093/intimm/dxh338 |
| Citation | Yao G, et al. (2006) Identification of core functional region of murine IL-4 using peptide phage display and molecular modeling. Int Immunol 18(1):19-29 |
| abstractText | Murine IL-4 is a pleiotropic cytokine with undefined core functional region for eliciting downstream signaling. We used molecular modeling to predict the binding sites recognized by an anti-IL-4-neutralizing mAb (11B.11) and peptide phage display to delineate their makeup. The results of these approaches were confirmed by site-directed mutagenesis analysis. The results suggest that the amino acid residues spanning from 79 to 86 (QRLFRAFR) on IL-4 are of the major binding site for 11B.11. Furthermore, the functional experiments demonstrate that the residues R80, R83 and R86, which are located in the helix C of murine IL-4, play a crucial role in binding to the IL-4R alpha-chain. Taken together, a new core functional region of murine IL-4 is identified, which provides new insight into the interaction between IL-4 and IL-4Ralpha. In addition, the results demonstrate that 11B.11 binds to a core functional region of murine IL-4, which prevents this cytokine from interacting with its cognate receptor. |
