| First Author | Yan QJ | Year | 2005 |
| Journal | Rejuvenation Res | Volume | 8 |
| Issue | 4 | Pages | 248-53 |
| PubMed ID | 16313224 | Mgi Jnum | J:105305 |
| Mgi Id | MGI:3614705 | Doi | 10.1089/rej.2005.8.248 |
| Citation | Yan QJ, et al. (2005) NANOG changes in mouse kidneys with age. Rejuvenation Res 8(4):248-53 |
| abstractText | NANOG is essential for mouse and human embryonic stem cell (ESC) pluripotency and selfrenewal. It is also expressed in several adult murine tissues as shown by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. However, human NANOG transcripts have been isolated from adult bone marrow (EST; GenBank accession no. BF893620). Here, we study the NANOG gene expression profile in isolated mouse renal papillary cells by Northern blot and RT-PCR. The whole RNA of mouse renal cells was obtained from fresh renal tissues, renal tissues infused by phosphate-buffered saline (PBS), and isolated renal papillary cells of mouse, respectively, as well as the renal papillary tissue from 18.5 days postcoitum (d.p.c.; fetal), 1-2-week-old (young), 1-8-month-old (adult), and 24-month-old (aging) mice. Our analysis shows that a very low expression level was detected in mouse renal tissues, and the renal papillary cells express more than other tissues as determined with Northern blot and RT-PCR. These data suggest that the kidney has its own cells expressing NANOG, and loss of NANOG expression occurs in an age-dependent manner in the kidney, either due to developmental factors or aging, particularly in renal papillary tissue. |
