| First Author | Chavez JA | Year | 2006 |
| Journal | Biochem Biophys Res Commun | Volume | 342 |
| Issue | 4 | Pages | 1218-22 |
| PubMed ID | 16516160 | Mgi Jnum | J:107074 |
| Mgi Id | MGI:3620271 | Doi | 10.1016/j.bbrc.2006.02.091 |
| Citation | Chavez JA, et al. (2006) The 47kDa Akt substrate associates with phosphodiesterase 3B and regulates its level in adipocytes. Biochem Biophys Res Commun 342(4):1218-22 |
| abstractText | We have previously described a novel putative 47kDa substrate for the protein kinase Akt (designated AS47) in 3T3-L1 adipocytes. In the present study, we have found by co-immunoprecipitation that AS47 was associated with cyclic nucleotide phosphodiesterase 3B (PDE3B) in lysates of 3T3-L1 adipocytes. The patterns of expression of AS47 and PDE3B upon 3T3-L1 adipocyte differentiation, among mouse tissues, and in adipocytes with and without the transcription factor C/EBPalpha were virtually coincident. Partial knockdown of AS47 in 3T3-L1 adipocytes with shRNA resulted in a similar reduction in PDE3B protein. These results indicate that AS47 exists in a complex with PDE3B in adipocytes and that the amount of AS47 protein regulates the amount of PDE3B. |
