| First Author | Datta SK | Year | 2006 |
| Journal | Immunity | Volume | 25 |
| Issue | 1 | Pages | 143-52 |
| PubMed ID | 16860763 | Mgi Jnum | J:113413 |
| Mgi Id | MGI:3686557 | Doi | 10.1016/j.immuni.2006.05.013 |
| Citation | Datta SK, et al. (2006) Vaccination with irradiated Listeria induces protective T cell immunity. Immunity 25(1):143-52 |
| abstractText | We evaluated gamma-irradiated Listeria monocytogenes as a killed bacterial vaccine, testing the hypothesis that irradiation preserves antigenic and adjuvant structures destroyed by traditional heat or chemical inactivation. Irradiated Listeria monocytogenes (LM), unlike heat-killed LM, efficiently activated dendritic cells via Toll-like receptors and induced protective T cell responses in mice. Like live LM, irradiated LM induced Toll-like-receptor-independent T cell priming. Cross-presentation of irradiated listerial antigens to CD8(+) T cells involved TAP- and proteasome-dependent cytosolic antigen processing. These results establish that killed LM can induce protective T cell responses, previously thought to require live infection. gamma-irradiation may be potentially applied to numerous bacterial vaccine candidates, and irradiated bacteria could serve as a vaccine platform for recombinant antigens derived from other pathogens, allergens, or tumors. |
