| First Author | Li X | Year | 2006 |
| Journal | Biochem Biophys Res Commun | Volume | 350 |
| Issue | 1 | Pages | 1-6 |
| PubMed ID | 16996026 | Mgi Jnum | J:114523 |
| Mgi Id | MGI:3689266 | Doi | 10.1016/j.bbrc.2006.08.188 |
| Citation | Li X, et al. (2006) Lactacystin inhibits 3T3-L1 adipocyte differentiation through induction of CHOP-10 expression. Biochem Biophys Res Commun 350(1):1-6 |
| abstractText | Hormonal induction triggers a cascade leading to the expression of CCAAT/enhancer-binding protein(C/EBP)alpha and peroxisome proliferator-activated receptor (PPAR) gamma, C/EBPalpha, and PPARgamma turns on series of adipocyte genes that give rise to the adipocyte phenotype. Previous findings indicate that C/EBPbeta, a transcriptional activator of the C/EBPalpha and PPARgamma genes, is rapidly expressed after induction, but lacks DNA-binding activity and therefore cannot activate transcription of the C/EBPalpha and PPARgamma genes early in the differentiation program. Acquisition of DNA-binding activity of C/EBPbeta occurs when CHOP-10, a dominant-negative form of C/EBP family members, is down-regulated and becomes hyperphosphorylated as preadipocytes traverse the G1-S checkpoint of mitotic clonal expansion. Evidences are presented in this report that lactacystin, a proteasome inhibitor, up-regulated the CHOP-10 expression, blocked the DNA-binding activity of C/EBPbeta, and subsequently inhibited MCE as well as adipocyte differentiation. |
