| First Author | Manzano-León N | Year | 2006 |
| Journal | Biochem Biophys Res Commun | Volume | 351 |
| Issue | 3 | Pages | 588-94 |
| PubMed ID | 17092488 | Mgi Jnum | J:115265 |
| Mgi Id | MGI:3691251 | Doi | 10.1016/j.bbrc.2006.10.077 |
| Citation | Manzano-Leon N, et al. (2006) Beta-adaptin: key molecule for microglial scavenger receptor function under oxidative stress. Biochem Biophys Res Commun 351(3):588-94 |
| abstractText | Scavenger receptors internalize chemically modified low density lipoprotein particles (ac-LDL) and other ligands through the process of receptor-mediated endocytosis. During this investigation using amyloid-beta as a natural ligand for the SR, we studied under a ligand-induced oxidative stress condition, changes in protein expression of several adaptor proteins important in the organization of the endocytic machinery in microglia and macrophages. Differential expression experiments of beta-adaptin, alpha-adaptin, SR-AI, and SR-BI in RAW (macrophages) and EOC (microglia) cells were performed according to dosage and exposure time to amyloid-beta. Our results show that according to dosage, amyloid-beta produces an oxidative stress state that importantly affects the availability of beta-adaptin. Under these conditions, RT-PCR assays show that beta-adaptin mRNA is normally synthesized, reason why protein translation or protein structure of beta-adaptin might be altered. These observations might have impact in the understanding of the mechanisms microglia employ to process amyloid-beta in the brain. |
