| First Author | Yang K | Year | 2006 |
| Journal | Cell Immunol | Volume | 243 |
| Issue | 2 | Pages | 90-5 |
| PubMed ID | 17324390 | Mgi Jnum | J:120726 |
| Mgi Id | MGI:3707901 | Doi | 10.1016/j.cellimm.2007.01.002 |
| Citation | Yang K, et al. (2006) Generation of HSP60-specific regulatory T cell and effect on atherosclerosis. Cell Immunol 243(2):90-5 |
| abstractText | Although CD4(+)CD25(+) regulatory T cells are pivotal in the suppression of autoimmunity, little is known about the effect of antigen-specific regulatory T cells on the formation of atheromatous plaques. Here, we describe the induction of heat-shock protein 60 (HSP60)-specific CD4(+)CD25(high) T cells by rapamycin (RPM)-treated immature dendritic cells in vitro and explore their effect on plaques in apolipoprotein E-deficient mice. Rapamycin-treated bone marrow-derived dendritic cells (DC) were immature, expressing a low level of co-stimulation factors CD86 and CD80. Naive CD4(+) T cells expressed high levels of CD25 and forkhead box P3 (Foxp3) after incubation with rapamycin-treated and HSP60-loaded DC and displayed moderate antigen-specific, IL-10-independent inhibitory function in vitro. After adoptive transfer, HSP60-specific CD4(+)CD25(high) T cells inhibited the formation of plaques, while ovalbumin-specific cells did not. These findings suggest that RPM-treated DC can induce antigen-specific CD4(+)CD25(high) Treg cells that have inhibitory activity in vitro and prevent the development of plaques in vivo. |
